New Fluorophores for Chemical Biology
New Fluorophores for Chemical Biology
Our laboratory is developing new fluorophores for applications in chemical biology. These scaffolds are being used to develop bioimaging reagents for proteins, small molecules, and post-translational modifications.
Evolution of Miniprotein Hosts
Evolution of Miniprotein Hosts
We are developing direct activity probes for protein phosphatases. These sensors can be used to profile phosphatase activity in human disease models.
Cellular Assays for Protein Solubility
Cellular Assays for Protein Solubility
Protein misfolding and aggregation plays a central role in numerous human diseases including Alzheimer’s, Parkinson’s, and Type II Diabetes. We have developed a sensitive luminescence-based assay that can be used to study this process in living cells as w
Endogenous Protein Phosphatase Assays
Endogenous Protein Phosphatase Assays
Our laboratory is developing new fluorophores for applications in chemical biology. These scaffolds are being used to develop bioimaging reagents for proteins, small molecules, and post-translational modifications.
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Meet the Group

Meet the Group

Back row from left to right: Frederik Broendsted, David Dimeglio, Yuchen He, and Ruwen Yin. Front row from left to right: Cliff Stains, Mehrdad Shadmehr, Shuo Liang, Tatiana Wirth, Yuan Fang, and Haoyu Sun.
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Research

Our laboratory focuses on the development and application of new tools for chemical biology:

 

 

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Selected Publications

  • Fang, Y.; Good, G.N.; Zhou, X. & Stains, C.I. Phosphinate-Containing Rhodol and Fluorescein Scaffolds for the Development of Bioprobes. Chem. Commun. 55, 5962-5965 (2019). DOI: 10.1039/C9CC02492A.
  • Zhou, X; Fang, Y.; Lesiak, L. & Stains, C.I. A Phosphinate‐Containing Fluorophore Capable of Selectively Inducing Apoptosis in Cancer Cells. ChemBioChem 20, 1712-1716 (2019). DOI: 10.1002/cbic.201800811.
  • Zhou, X.; Lesiak, L.; Lai, R.; Beck, J.R.; Zhao, J.; Elowsky, C.G.; Li, H. & Stains, C.I. Chemoselective Alteration of Fluorophore Scaffolds as a Strategy for the Development of Ratiometric Chemodosimeters. Angew. Chem. Int. Ed. 56, 4197-4200 (2017). DOI: 10.1002/anie.201612628